Rat NF-L ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-81185

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat NF-L in samples. No significant cross-reactivity or interference between Rat NF-L and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.36%

Inter-Assay Precision (Precision between assays) CV% 4.4%

Recovery for Rat NF-L ELISA Kit (Colorimetric)

Recovery

87-102%

Linearity

Rat NF-L ELISA Kit (Colorimetric)

Scientific Data Images for Rat NF-L ELISA Kit (Colorimetric)

Rat NF-L ELISA Kit (Colorimetric)

ELISA: Rat NF-L ELISA Kit (Colorimetric) [NBP2-81185] -

ELISA: Rat NF-L ELISA Kit (Colorimetric) [NBP2-81185] - Standard Curve Reference
Rat NF-L ELISA Kit (Colorimetric)

ELISA: Rat NF-L ELISA Kit (Colorimetric) [NBP2-81185] -

ELISA: Rat NF-L ELISA Kit (Colorimetric) [NBP2-81185] - Image from a verified customer review.

Kit Contents for Rat NF-L ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: NF-L

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for Rat NF-L ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat NF-L ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  • Rat NF-L ELISA Kit (Colorimetric)
    Name: Anonymous
    Sample Tested: Blood plasma
    Verified Customer | Posted 04/17/2024
    Rat NF-L ELISA Kit (Colorimetric) NBP2-81185

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