NF-M Antibody (24GB5825)

Novus Biologicals | Catalog # NBP3-51744

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 24GB5825
View all NF-M Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human NF-M (Uniprot: P07197)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NF-M Antibody (24GB5825)

NF-M Antibody (24GB5825)

Flow Cytometry: NF-M Antibody (24GB5825)[NBP3-51744] -

Flow cytometric analysis of NF-Mexpression in HeLa cells using NF-M antibody (1:2,000). Green, isotype control; red, neurofilament medium chain.
NF-M Antibody (24GB5825)

Western Blot: NF-M Antibody (24GB5825)[NBP3-51744] -

Western blotting analysis using NF-M antibody (1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
NF-M Antibody (24GB5825)

Western Blot: NF-M Antibody (24GB5825)[NBP3-51744] -

Western blotting analysis using NF-M antibody (1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for NF-M Antibody (24GB5825)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NF-M

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Medium Chain

Alternate Names

NEF3, NEFM, NFM

Gene Symbol

NEFM

Additional NF-M Products

Product Documents for NF-M Antibody (24GB5825)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NF-M Antibody (24GB5825)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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