Rat NF-M ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-78760

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids. (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL
View all NF-M ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat NEF3 in samples. No Significant cross-reactivity or interference between Rat NEF3 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.21%

Inter-Assay Precision (Precision between assays) CV% 4.77%

Recovery for Rat NF-M ELISA Kit (Colorimetric)

Recovery

83-101%

Linearity

Rat NF-M ELISA Kit (Colorimetric)

Scientific Data Images for Rat NF-M ELISA Kit (Colorimetric)

Rat NF-M ELISA Kit (Colorimetric)

ELISA: Rat NF-M ELISA Kit (Colorimetric) [NBP2-78760] -

ELISA: Rat NF-M ELISA Kit (Colorimetric) [NBP2-78760] - Standard Curve Reference

Kit Contents for Rat NF-M ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: NF-M

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Medium Chain

Alternate Names

NEF3, NEFM, NFM

Gene Symbol

NEFM

UniProt

P12839 (Rat)

Additional NF-M Products

Product Documents for Rat NF-M ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat NF-M ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs

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