NFkB p105/p50 Antibody (5D10D11)

Novus Biologicals | Catalog # NBP2-22178

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5D10D11
View all NFkB p105/p50 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human NFkB p105/p50 expressed in E. Coli.

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50/105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFkB p105/p50 Antibody (5D10D11)

Western Blot: NFkB p105/p50 Antibody (5D10D11)BSA Free [NBP2-22178]

Western Blot: NFkB p105/p50 Antibody (5D10D11)BSA Free [NBP2-22178]

Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis using NFkB p105/p50 mouse mAb against K562 (1), Jurkat (2), A431 (3), Hela (4), THP-1 (5) and MCF-7 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody (5D10D11) - BSA Free [NBP2-22178]

Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody (5D10D11) - BSA Free [NBP2-22178]

Immunocytochemistry/Immunofluorescence: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of U251 cells using NFkB p105/p50 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (5D10D11) - BSA Free [NBP2-22178]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (5D10D11) - BSA Free [NBP2-22178]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of paraffin-embedded human intima cancer tissues (left) and human bladder cancer tissues (right) using NFkB p105/p50 mouse mAb with DAB staining.
Flow Cytometry: NFkB p105/p50 Antibody (5D10D11) - BSA Free [NBP2-22178]

Flow Cytometry: NFkB p105/p50 Antibody (5D10D11) - BSA Free [NBP2-22178]

Flow Cytometry: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Flow cytometric analysis of MCF-7 cells using NFkB p105/p50 mouse mAb (green) and negative control (purple).
Western Blot: NFkB p105/p50 Antibody (5D10D11)BSA Free [NBP2-22178]

Western Blot: NFkB p105/p50 Antibody (5D10D11)BSA Free [NBP2-22178]

Western Blot: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Analysis of A431 (A) and HeLa (B) cell lysate using NFkB p105/p50 (NBP2-22178) antibody at 1:500.
ELISA: NFkB p105/p50 Antibody (5D10D11) - BSA Free [NBP2-22178]

ELISA: NFkB p105/p50 Antibody (5D10D11) - BSA Free [NBP2-22178]

ELISA: NFkB p105/p50 Antibody (5D10D11) [NBP2-22178] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Applications for NFkB p105/p50 Antibody (5D10D11)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFkB p105/p50

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

DKFZp686C01211, DNA binding factor KBF1, DNA-binding factor KBF1, EBP-1, KBF1, NF-kappaB, NF-kappa-B, NF-kappabeta, NFKB-p105, NFKB-p50, nuclear factor kappa-B DNA binding subunit, nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151, p105, p50

Gene Symbol

NFKB1

Additional NFkB p105/p50 Products

Product Documents for NFkB p105/p50 Antibody (5D10D11)

Certificate of Analysis

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Product Specific Notices for NFkB p105/p50 Antibody (5D10D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NFkB p105/p50 Antibody (5D10D11)

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Protocols

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FAQs

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