NFRKB Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85095

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NFRKB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KSSSGVLLVSSPTMPHLGTMLSPASSQTAPSSQAAARVVSHSGSAGLSQVRVVAQPSLPAVPQQSGGPAQTLPQMPAGPQIRVPATATQTKVVPQTVMATVPVKAQTTAATV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFRKB Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NFRKB Antibody [NBP1-85095]

Immunocytochemistry/ Immunofluorescence: NFRKB Antibody [NBP1-85095]

Immunocytochemistry/Immunofluorescence: NFRKB Antibody [NBP1-85095] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Immunohistochemistry-Paraffin: NFRKB Antibody [NBP1-85095]

Immunohistochemistry-Paraffin: NFRKB Antibody [NBP1-85095]

Immunohistochemistry-Paraffin: NFRKB Antibody [NBP1-85095] - Staining of human gallbladder shows moderate nuclear positivity in glandular cells.
NFRKB Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: NFRKB Antibody - BSA Free [NBP1-85095]

Chromatin Immunoprecipitation-exo-Seq: NFRKB Antibody - BSA Free [NBP1-85095]

ChIP-Exo-Seq composite graph for Anti-NFRKB (NBP1-85095) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for NFRKB Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFRKB

NFRKB (nuclear factor related to kappa-B-binding protein) was first identified as a factor that could bind a variant NF-kappa B site in the interleukin-2 receptor alpha (IL-2R alpha) gene. NFRKB appears to be a transcription factor central to immune cell development. Evidence of this comes from the identification of NFRKB as an amplified gene in several cases of acute myeloid leukemia. Additionally, in another clinical case, a partial deletion of the chromosomal region that includes NFRKB is shown to be associated with combined cellular immunodeficiency.

Alternate Names

DKFZp547B2013, DNA-binding protein R kappa-B, INO80 complex subunit G, INO80GDNA-binding protein R kappa B, nuclear factor related to kappaB binding protein, nuclear factor related to kappa-B-binding protein

Gene Symbol

NFRKB

Additional NFRKB Products

Product Documents for NFRKB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NFRKB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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