NFYC Antibody (1D3) - Azide and BSA Free

Novus Biologicals | Catalog # H00004802-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 1D3

Format

Azide and BSA Free
View all NFYC Primary Antibodies »

Product Specifications

Immunogen

NFYC (AAH05003, 14 a.a. ~ 113 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DAQQSLQSFWPRVMEEIRNLTVKDFRVQELPLARIKKIMKLDEDVKMISAEAPVLFAKAAQIFITELTLRAWIHTEDNKRRTLQRNDIAMAITKFDQFDF

Specificity

NFYC - nuclear transcription factor Y, gamma

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NFYC Antibody (1D3) - Azide and BSA Free

Western Blot: NFYC Antibody (1D3) [H00004802-M01]

Western Blot: NFYC Antibody (1D3) [H00004802-M01]

Western Blot: NFYC Antibody (1D3) [H00004802-M01] - Analysis of NFYC expression in transfected 293T cell line by NFYC monoclonal antibody (M01), clone 1D3.Lane 1: NFYC transfected lysate(37.2 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NFYC Antibody (1D3) [H00004802-M01]

Immunocytochemistry/ Immunofluorescence: NFYC Antibody (1D3) [H00004802-M01]

Immunocytochemistry/Immunofluorescence: NFYC Antibody (1D3) [H00004802-M01] - Analysis of monoclonal antibody to NFYC on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NFYC Antibody (1D3) [H00004802-M01]

Immunohistochemistry-Paraffin: NFYC Antibody (1D3) [H00004802-M01]

Immunohistochemistry-Paraffin: NFYC Antibody (1D3) [H00004802-M01] - Analysis of monoclonal antibody to NFYC on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Western Blot: NFYC Antibody (1D3) [H00004802-M01]

Western Blot: NFYC Antibody (1D3) [H00004802-M01]

Western Blot: NFYC Antibody (1D3) [H00004802-M01] - Analysis of NFYC over-expressed 293 cell line, cotransfected with NFYC Validated Chimera RNAi ( Cat # H00004802-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYC monoclonal antibody (M01), clone 1D3 (Cat # H00004802-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: NFYC Antibody (1D3) [H00004802-M01]

ELISA: NFYC Antibody (1D3) [H00004802-M01]

ELISA: NFYC Antibody (1D3) [H00004802-M01] - Detection limit for recombinant GST tagged NFYC is 3 ng/ml as a capture antibody.

Applications for NFYC Antibody (1D3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFYC

This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.

Alternate Names

C subunit, nuclear transcription factor Y, gamma

Entrez Gene IDs

4802 (Human)

Gene Symbol

NFYC

OMIM

605344 (Human)

UniProt

AAH05003 (Human)

Additional NFYC Products

Product Documents for NFYC Antibody (1D3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NFYC Antibody (1D3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NFYC Antibody (1D3) - Azide and BSA Free

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Protocols

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FAQs

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