NGFI-B alpha/Nur77/NR4A1 Antibody

Novus Biologicals | Catalog # NBP2-19613

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (95%), Canine (96%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NGFI-B alpha/Nur77/NR4A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NGFI-B alpha/Nur77/NR4A1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NGFI-B alpha/Nur77/NR4A1 Antibody

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUR77 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613] - A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613] - Western Blot analysis of NR4A1 expression in transfected 293T cell line by NR4A1 polyclonal antibody. A: Non-transfected lysate. B: NR4A1 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.
NGFI-B alpha/Nur77/NR4A1 Antibody

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NBP2-19613] -

Untreated and treated Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NUR77 antibody (NBP2-19613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for NGFI-B alpha/Nur77/NR4A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NGFI-B alpha/Nur77/NR4A1

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Long Name

Nerve Growth Factor-induced Protein I-B alpha/Nuclear Receptor Subfamily 4, Group A, Member 1

Alternate Names

HMR, NAK1, NGFIB alpha, NR4A1, Nur77, TR3

Gene Symbol

NR4A1

UniProt

P22736 (Human)

Additional NGFI-B alpha/Nur77/NR4A1 Products

Product Documents for NGFI-B alpha/Nur77/NR4A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NGFI-B alpha/Nur77/NR4A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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