Catalog Number: 3439
Chemical Name: 1-Hexadecyl-2-methyl-3-(phenylmethyl)-1H-imidazolium iodide
Biological Activity
Eukaryotic elongation factor 2 (eEF-2) kinase (CaMK III) inhibitor (IC50 = 60 nM) that displays 125-fold, > 1300-fold and > 1500-fold selectivity over PKC, PKA and CaMK II respectively. Decreases the viability of a variety of cancer cell lines (IC50 values are 0.7 - 4.8 μM) and blocks G1/S cell cycle progression. Shown to induce eEF-2 phosphorylation in cancer cells. Also an effective antibacterial agent; inhibits histidine protein kinase in vitro (IC50 = 6.6 μM) and in vivo.
Technical Data
  • M.Wt:
    524.56
  • Formula:
    C27H45IN2
  • Solubility:
    Soluble to 100 mM in DMSO and to 75 mM in ethanol
  • Storage:
    Desiccate at -20°C
  • CAS No:
    278603-08-0
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. 1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) induces phosphorylation of eukaryotic elongation factor-2 (eEF2): a cautionary note on the anticancer mechanism of an eEF2 kinase inhibitor.
    Chen
    J.Biol.Chem., 2011;286:43951
  2. Identification and characterization of an inhibitor of eukaryotic elongation factor 2 kinase against human cancer cell lines.
    Arora et al.
    Cancer Res., 2003;63:6894
  3. P-glycoprotein mediates resistance to histidine kinase inhibitors.
    Arora et al.
    Mol.Pharmacol., 2004;66:460
  4. Antibacterial agents that inhibit histidine protein kinase YycG of Bacillus subtilis.
    Yamamoto et al.
    Biosci.Biotechnol.Biochem., 2001;65:2306
Citations:

The citations listed below are publications that use Tocris products. Selected citations for NH 125 include:

3 Citations: Showing 1 - 3
Filter your results:

  1. Identification of an Antimicrobial Agent Effective against Methicillin-Resistant Staphylococcus aureus Persisters Using a Fluorescence-Based Screening Strategy.
    Authors: Kim Et al.
    PLoS One 2015;10:e0127640
  2. Integrated regulation of autophagy and apoptosis by EEF2K controls cellular fate and modulates the efficacy of curcumin and velcade against tumor cells.
    Authors: Cheng Et al.
    Autophagy 2013;9:208
  3. Mechanistic insight into inhibition of two-component system signaling.
    Authors: Francis Et al.
    Medchemcomm 2013;4:269

Have you used NH 125?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.

Order Details

Note:

Contact Information

Calculators

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

R&D Systems Guarantee

Feedback

Customer Information