Nicalin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81002

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nicalin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RLLDFSYEQYQKALRQSAGAVVIILPRAMAAVPQDVVRQFMEIEPEMLAMETAVPVYFAVEDEALLSIYKQTQAASASQGSASAAEVLLRTATANGFQMVTSGVQSKAVSDWLIASVE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261),Analysis of mouse brain lysate. Image from verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nicalin Antibody - BSA Free

Western Blot: Nicalin Antibody [NBP1-81002]

Western Blot: Nicalin Antibody [NBP1-81002]

Western Blot: Nicalin Antibody [NBP1-81002] - Analysis of mouse brain lysate. Image from verified customer review.
Immunohistochemistry-Paraffin: Nicalin Antibody [NBP1-81002]

Immunohistochemistry-Paraffin: Nicalin Antibody [NBP1-81002]

Immunohistochemistry-Paraffin: Nicalin Antibody [NBP1-81002] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Applications for Nicalin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Western blot data on mouse from customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-81002 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicalin

Nicastrin is an integral part of the Alzheimers associated gamma-secretase complex. Nicalin, a Nicastrin-like protein, is not associated with gamma-secretase but rather interacts with NOMO (Nodal modulator). Nodals are TGFbeta signaling factors that control various cell fate decisions during embryonic body planning stages in vertebrate development. The Nicalin/NOMO complex acts to regulate the Nodal signaling factors during gastrulation. This regulation most often affects the development of the axial mesoderm. Nodal signaling is an important factor for melanoma cell invasiveness and tumorigenicity and inhibition of this signal can promote melanoma cells reverting back toward a melanocyte phenotype.

Alternate Names

Ncl-1, NCLN, NET59

Entrez Gene IDs

56926 (Human)

Gene Symbol

NCLN

UniProt

Q969V3 (Human)

Additional Nicalin Products

Product Documents for Nicalin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Nicalin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nicalin Antibody - BSA Free

Customer Reviews for Nicalin Antibody - BSA Free (1)

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Customer Images

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  • Name: Margaret Harvey
    Application: Western Blot
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 12/03/2014
    Nicalin antibody on mouse brain lysate
    Nicalin Antibody - BSA Free NBP1-81002

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Protocols

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FAQs

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