Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - BSA Free

Novus Biologicals | Catalog # NBP3-43572

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 12H2

Format

BSA Free
View all Nicotinic Acetylcholine R beta 2/CHRNB2 Primary Antibodies »

Product Specifications

Immunogen

The original antibody was generated by immunizing female BALB/c mice with human alpha4beta2 receptor protein purified in n-dodecyl-beta-D-maltopyranoside (DDM). (Uniprot# P17787)

Specificity

This antibody recognizes human alpha4beta2 receptor protein. The AChR responds by an extensive change in conformation after binding acetylcholine, that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - BSA Free

Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - BSA Free Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - BSA Free

Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - BSA Free [NBP3-43572] -

Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - BSA Free [NBP3-43572] - Immunofluorescence analysis of paraformaldehyde fixed Kelly cells on coverslips stained with the chimeric version of this antibody, NBP3-43573 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43573, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Applications for Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R beta 2/CHRNB2

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. After binding Acetylcholine, the Nicotinic Acetylcholine Receptor (AChR) responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Neuronal AChR seems to be composed of two different type of subunits: alpha and beta.

Long Name

Nicotinic Acetylcholine Receptor beta 2

Alternate Names

Cholinergic Receptor Nicotinic Beta 2 Subunit, CHRNB2, EFNL3, NAChRB2, Neuronal Nicotinic Acetylcholine R beta 2

Gene Symbol

CHRNB2

Additional Nicotinic Acetylcholine R beta 2/CHRNB2 Products

Product Documents for Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Nicotinic Acetylcholine Receptor beta 2 Antibody (12H2) - BSA Free

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  • Q: I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

    A:

    A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

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