Nidogen-2 Antibody (4G8) - Azide and BSA Free

Novus Biologicals | Catalog # H00022795-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4G8

Format

Azide and BSA Free
View all Nidogen-2 Primary Antibodies »

Product Specifications

Immunogen

NID2 (NP_031387.2, 1276 a.a. ~ 1375 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFSIVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

Specificity

Reacts with nidogen 2 (osteonidogen).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Novus Biologicals Mouse Nidogen-2 Antibody (4G8) - Azide and BSA Free (H00022795-M01) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Nidogen-2 Antibody (4G8) - Azide and BSA Free

Western Blot: Nidogen-2 Antibody (4G8) [H00022795-M01]

Western Blot: Nidogen-2 Antibody (4G8) [H00022795-M01]

Western Blot: Nidogen-2 Antibody (4G8) [H00022795-M01] - Analysis of NID2 expression in transfected 293T cell line by NID2 monoclonal antibody (M01), clone 4G8.Lane 1: NID2 transfected lysate (Predicted MW: 107.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Nidogen-2 Antibody (4G8) [H00022795-M01] - Detection limit for recombinant GST tagged NID2 is 1 ng/ml as a capture antibody.

Applications for Nidogen-2 Antibody (4G8) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nidogen-2

Basement membranes, which are composed of type IV collagens (see MIM 120130), laminins (see LAMC1; MIM 150290), perlecan (HSPG2; MIM 142461), and nidogen (see NID1; MIM 131390), are thin pericellular protein matrices that control a large number of cellular activities, including adhesion, migration, differentiation, gene expression, and apoptosis.[supplied by OMIM]

Alternate Names

NID2, Nidogen2, Osteonidogen

Entrez Gene IDs

22795 (Human)

Gene Symbol

NID2

UniProt

NP_031387 (Human)

Additional Nidogen-2 Products

Product Documents for Nidogen-2 Antibody (4G8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nidogen-2 Antibody (4G8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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