Niemann-Pick C1 Antibody (4H2) - Azide and BSA Free

Novus Biologicals | Catalog # H00004864-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H2

Format

Azide and BSA Free
View all Niemann-Pick Type C1/NPC1 Primary Antibodies »

Product Specifications

Immunogen

NPC1 (AAH63302, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPK

Localization

Late endosome and Lysosome membrane; Single-pass membrane protein.

Specificity

NPC1 - Niemann-Pick disease, type C1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Niemann-Pick C1 Antibody (4H2) - Azide and BSA Free

Western Blot: Niemann-Pick C1 Antibody (4H2) [H00004864-M02]

Western Blot: Niemann-Pick C1 Antibody (4H2) [H00004864-M02]

Western Blot: Niemann-Pick C1 Antibody (4H2) [H00004864-M02] - NPC1 monoclonal antibody (M02), clone 4H2. Analysis of NPC1 expression in HeLa.
ELISA: Niemann-Pick C1 Antibody (4H2) [H00004864-M02]

ELISA: Niemann-Pick C1 Antibody (4H2) [H00004864-M02]

ELISA: Niemann-Pick C1 Antibody (4H2) [H00004864-M02] - Detection limit for recombinant GST tagged NPC1 is approximately 0.03ng/ml as a capture antibody.

Applications for Niemann-Pick C1 Antibody (4H2) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Niemann-Pick Type C1/NPC1

This gene was identified as the gene that when mutated, results in Niemann-Pick C disease. The encoded protein is a putative integral membrane protein that contains motifs consistent with a role in intracellular transport of cholesterol to post-lysosomal destinations.

Alternate Names

NPC1, SLC65A1

Entrez Gene IDs

4864 (Human)

Gene Symbol

NPC1

OMIM

257220 (Human)

UniProt

AAH63302 (Human)

Additional Niemann-Pick Type C1/NPC1 Products

Product Documents for Niemann-Pick C1 Antibody (4H2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Niemann-Pick C1 Antibody (4H2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Niemann-Pick C1 Antibody (4H2) - Azide and BSA Free

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FAQs for Niemann-Pick C1 Antibody (4H2) - Azide and BSA Free

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  • Q: I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?

    A: Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01P, but this antibody is yet to be established for IHC application. However, if you would like to try this antibody for IHC-P application you would qualify for our Innovators Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product. Read more about Innovators Reward Program.

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