Niemann-Pick type C2 Antibody (012) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90209

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 012 expressed in HEK293

Format

Azide and BSA Free
View all Niemann-Pick Type C2/NPC2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Niemann-Pick type C2 (Accession#: NP_006423.1; Met1-Leu151).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Niemann-Pick type C2 Antibody (012) - Azide and BSA Free

Western Blot: Niemann-Pick type C2 Antibody (012) [NBP2-90209]

Western Blot: Niemann-Pick type C2 Antibody (012) [NBP2-90209]

Western Blot: Niemann-Pick type C2 Antibody (012) [NBP2-90209] - Anti-NPC2 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: NIH-3T3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique.
Immunoprecipitation: Niemann-Pick type C2 Antibody (012) [NBP2-90209]

Immunoprecipitation: Niemann-Pick type C2 Antibody (012) [NBP2-90209]

Immunoprecipitation: Niemann-Pick type C2 Antibody (012) [NBP2-90209] - NPC2-His was immunoprecipitated using: Lane A:0.5 mg NIH-3T3 Whole Cell Lysate. 2 uL anti-NPC2-His rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NPC2-His rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique.

Applications for Niemann-Pick type C2 Antibody (012) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Niemann-Pick Type C2/NPC2

Niemann-Pick type C2 encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. [provided by RefSeq]

Alternate Names

EDDM1, Epididymal Protein 1, HE1, NiemannPick Type C2, NPC2

Gene Symbol

NPC2

Additional Niemann-Pick Type C2/NPC2 Products

Product Documents for Niemann-Pick type C2 Antibody (012) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Niemann-Pick type C2 Antibody (012) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Niemann-Pick type C2 Antibody (012) - Azide and BSA Free

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  • Q: I'm looking for a Niemann-Pick type1 or 2 antibody to stain mouse heart section embedded in paraffin. I would like to label these sections with another primary antibody to show colocalisation of my protein of interest. I would like to know if you have a rabbit anti-Niemann-Pick antibody that works well for fluorescence immunohistochemistry?

    A:

    Niemann-Pick C1 Antibody (NB400-148) (0.1 ml) is one of our best sellers and this antibody has been cited in at least 17 peer reviewed research publications in journals of high repute. We have only one Niemann-Pick type C2 antibody with catalog # H00010577-D01PInnovators Reward Program.

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