Ninein Antibody

Novus Biologicals | Catalog # NB100-74631

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1850 and 1900 of human Ninein using the numbering given in entry NP_891991.1 (GeneID 51199).

Marker

Centrosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

243 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ninein Antibody

Western Blot: Ninein Antibody [NB100-74631]

Western Blot: Ninein Antibody [NB100-74631]

Ninein-Antibody-Western-Blot-NB100-74631-img0003.jpg
Western Blot: Ninein Antibody [NB100-74631]

Western Blot: Ninein Antibody [NB100-74631]

Western Blot: Ninein Antibody [NB100-74631] - Detection of Human and Mouse NINEIN on HeLa whole cell lysate using NB100-74631. NINEIN was also immunoprecipitated by another rabbit anti-NINEIN antibody.

Applications for Ninein Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 1 using NB100-74631 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ninein

This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.

Alternate Names

Glycogen synthase kinase 3 beta-interacting protein, GSK3B-interacting protein, hNinein, KIAA1565, ninein, ninein (GSK3B interacting protein), ninein centrosomal protein

Entrez Gene IDs

51199 (Human); 18080 (Mouse)

Gene Symbol

NIN

UniProt

Q8N4C6 (Human)

Additional Ninein Products

Product Documents for Ninein Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Ninein Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ninein Antibody

Customer Reviews for Ninein Antibody (1)

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Customer Images

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  • Ninein Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Adult small intestine
    Species: Mouse
    Verified Customer | Posted 08/16/2021
    smear preparation of cells fixed with 2% paraformaldehyde/lysine/periodate (PLP) and stained for ZO-1 (green) and with this ninein antibody (red). No specific signal is seen.
    The sample is a smear (cytology-like preps) of mouse small intestinal epithelial cells fixed with 2% paraformaldehyde/lysine/periodate (PLP; McLean and Nakane. J Histochem Cytochem, 1974). Results were similar after fixation with -20°C methanol. After permeabilizing with 0.1% saponin (PLP-fixed only) and blocking with 5% donkey serum (in PBS), slides were incubated with antibodies at the dilutions indicated below and a rat monoclonal anti-ZO-1 (tight junction protein) antibody (clone R40.76) for 2 hours at room temperature. After washing, species-specific secondary antibodies (cross-adsorbed) produced in goat were applied.
    Ninein Antibody NB100-74631
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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