NKCC2/SLC12A1 Antibody (4H4) - Azide and BSA Free

Novus Biologicals | Catalog # H00006557-M03

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H4

Format

Azide and BSA Free
View all NKCC2/SLC12A1 Primary Antibodies »

Product Specifications

Immunogen

SLC12A1 (NP_000329.1, 80 a.a. ~ 172 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQA

Specificity

SLC12A1 - solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (4H4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NKCC2/SLC12A1 Antibody (4H4) - Azide and BSA Free

Western Blot: NKCC2/SLC12A1 Antibody (4H4) [H00006557-M03]

Western Blot: NKCC2/SLC12A1 Antibody (4H4) [H00006557-M03]

Western Blot: NKCC2/SLC12A1 Antibody (4H4) [H00006557-M03] - Analysis of SLC12A1 expression in transfected 293T cell line by SLC12A1 monoclonal antibody (M03), clone 4H4. Lane 1: SLC12A1 transfected lysatE (46.5 KDa). Lane 2: Non-transfected lysate.

Applications for NKCC2/SLC12A1 Antibody (4H4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NKCC2/SLC12A1

The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. [provided by RefSeq]

Long Name

Sodium/Potassium/Chloride Transporter NKCC2

Alternate Names

BSC1, SLC12A1

Entrez Gene IDs

6557 (Human)

Gene Symbol

SLC12A1

UniProt

NP_000329

Additional NKCC2/SLC12A1 Products

Product Documents for NKCC2/SLC12A1 Antibody (4H4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NKCC2/SLC12A1 Antibody (4H4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NKCC2/SLC12A1 Antibody (4H4) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review NKCC2/SLC12A1 Antibody (4H4) - Azide and BSA Free and earn rewards!

Have you used NKCC2/SLC12A1 Antibody (4H4) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies