NM23-H1 Antibody (1D7) - Azide and BSA Free

Novus Biologicals | Catalog # H00004830-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D7

Format

Azide and BSA Free
View all NM23-H1 Primary Antibodies »

Product Specifications

Immunogen

NME1 (NP_000260, 43 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE

Specificity

NME1 - non-metastatic cells 1, protein (NM23A) expressed in

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NM23-H1 Antibody (1D7) - Azide and BSA Free

Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02]

Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02]

Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02]

Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02]

Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NM23-H1 Antibody (1D7) [H00004830-M02]

Immunohistochemistry-Paraffin: NM23-H1 Antibody (1D7) [H00004830-M02]

Immunohistochemistry-Paraffin: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02]

Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02]

Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in HeLa.
Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02]

Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02]

Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in different cell lines.
Immunoprecipitation: NM23-H1 Antibody (1D7) [H00004830-M02]

Immunoprecipitation: NM23-H1 Antibody (1D7) [H00004830-M02]

Immunoprecipitation: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 transfected lysate using anti-NME1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NME1 MaxPab rabbit polyclonal antibody.
ELISA: NM23-H1 Antibody (1D7) [H00004830-M02]

ELISA: NM23-H1 Antibody (1D7) [H00004830-M02]

ELISA: NM23-H1 Antibody (1D7) [H00004830-M02] - Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.

Applications for NM23-H1 Antibody (1D7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NM23-H1

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Long Name

Non-metastatic Protein 23 Homolog 1

Alternate Names

GAAD, NDKA, NDPKA, NM23A, NM23H1, NME1

Entrez Gene IDs

4830 (Human)

Gene Symbol

NME1

UniProt

NP_000260 (Human)

Additional NM23-H1 Products

Product Documents for NM23-H1 Antibody (1D7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NM23-H1 Antibody (1D7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for NM23-H1 Antibody (1D7) - Azide and BSA Free

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Protocols

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FAQs

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