NMDAR2C Antibody - N-terminal - Azide and BSA Free

Novus Biologicals | Catalog # NB300-107

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all GRIN2C/NMDAR2C Primary Antibodies »

Product Specifications

Immunogen

Fusion protein from the N-terminal region of the NMDAR2C subunit. Accession # Q00961

Localization

Cerebellum

Specificity

Specific for endogenous levels of the ~140 kDa NR2C subunit of the NMDA receptor. Also labels the ~180 kDa NR2A and the ~180 kDa NR2B subunits of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the immunogen that was used to generate the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for NMDAR2C Antibody - N-terminal - Azide and BSA Free

Western Blot: NMDAR2C AntibodyAzide and BSA Free [NB300-107]

Western Blot: NMDAR2C AntibodyAzide and BSA Free [NB300-107]

Western Blot: NMDA R, NR2C Subunit Antibody [NB300-107]

Applications for NMDAR2C Antibody - N-terminal - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

3 ul per 200 ug lysate

Simple Western

1:25

Western Blot

1:1000
Application Notes

This antibody cross reacts with both NR2A and NR2B, as such, results in immunohistochemistry are varied.
See Simple Western Antibody Database for Simple Western validation: Tested in Brain, separated by Size, antibody dilution of 1:25

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute with 50 uL PBS to desired concentration.

Formulation

Lyophilized

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GRIN2C/NMDAR2C

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2C

Alternate Names

NMDA R, NR2C Subunit, NMDAR2C, NR2C

Entrez Gene IDs

2905 (Human); 14813 (Mouse); 24411 (Rat)

Gene Symbol

GRIN2C

UniProt

Q00961 (Rat)

Additional GRIN2C/NMDAR2C Products

Product Documents for NMDAR2C Antibody - N-terminal - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NMDAR2C Antibody - N-terminal - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NMDAR2C Antibody - N-terminal - Azide and BSA Free

Customer Reviews for NMDAR2C Antibody - N-terminal - Azide and BSA Free

There are currently no reviews for this product. Be the first to review NMDAR2C Antibody - N-terminal - Azide and BSA Free and earn rewards!

Have you used NMDAR2C Antibody - N-terminal - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for NMDAR2C Antibody - N-terminal - Azide and BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: We have a note from 2005 on our internal datasheet from Novus stating that this antibody has only been tested for IHC on frozen tissue but I see that the Novus datasheet now lists IHC-P. Can you please confirm whether the antibody has been tested on paraffin-embedded tissue? If yes, is there a specific protocol, in particular for antigen retrieval?

    A: NB300-107 has been validated in mouse for IHC-frozen, but not paraffin embedded sections yet.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies