Mouse nNOS ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-80253

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

0.094 ng/mL

Assay Range

0.156-10 ng/mL
View all nNOS ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse NOS1/nNOS in samples. No significant cross-reactivity or interference between Mouse NOS1/nNOS and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.73%

Inter-Assay Precision (Precision between assays) CV% 5.22%

Recovery for Mouse nNOS ELISA Kit (Colorimetric)

Recovery

85-99%

Linearity

Mouse nNOS ELISA Kit (Colorimetric)

Scientific Data Images for Mouse nNOS ELISA Kit (Colorimetric)

Mouse nNOS ELISA Kit (Colorimetric)

ELISA: Mouse nNOS ELISA Kit (Colorimetric) [NBP2-80253] -

ELISA: Mouse nNOS ELISA Kit (Colorimetric) [NBP2-80253] - Standard Curve Reference

Kit Contents for Mouse nNOS ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: nNOS

NOS (nitric oxide synathase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane and at increased level of Ca2+, nNOS is trasnlocated to cytoplasm. In cytoplasm, nNOS is dephosphorylated by calcineurin which initiates NO production (2). A phosphorylation by PKA and PKC on nNOS leads down-regulation of NO production.

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Gene Symbol

NOS1

Additional nNOS Products

Product Documents for Mouse nNOS ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse nNOS ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Oxidative Stress
Redox Enzymes

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