Nogo-A, also known as Reticulon 4 (RTN4) is a transmembrane protein that is mainly expressed in oligodendrocytes of the central nervous system but is also reported in fibroblasts, dorsal root ganglion neurons, macrophages, and myoblasts. Binding of the Nogo66 region to the Nogo Receptor/p75 complex on axons induces growth cone collapse and blocks neurite outgrowth. Nogo-A interactions with the Nogo-B receptor (NgBR/NUS1) can regulate vascular remodeling, inhibit neuronal differentiation, and promote glial formation from neural progenitors.
Nogo-A Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-41071
![Western Blot: Nogo-A AntibodyBSA Free [NBP2-41071]](https://resources.rndsystems.com/images/products/Nogo-A-Antibody-Western-Blot-NBP2-41071-img0001.jpg "Western Blot: Nogo-A AntibodyBSA Free [NBP2-41071]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 19 amino acid synthetic peptide from near the center of human NogoA. The immunogen is located within amino acids 200 - 250 of NogoA. Amino Acid Squence: DLKEQPGNTISAGQEDFPS
Specificity
At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA and NogoE.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
~180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nogo-A Antibody - BSA Free
Western Blot: Nogo-A AntibodyBSA Free [NBP2-41071]
Western Blot: Nogo-A Antibody [NBP2-41071] - Analysis of NogoA in human brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 ug/mL.![Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41071]](https://resources.rndsystems.com/images/products/Nogo-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41071-img0007.jpg "Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41071]")
Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41071]
Immunocytochemistry/Immunofluorescence: Nogo-A Antibody [NBP2-41071] - Staining of NogoA in mouse brain tissue with NogoA Antibodyat 20 ug/mL.![Immunohistochemistry-Paraffin: Nogo-A Antibody - BSA Free [NBP2-41071]](https://resources.rndsystems.com/images/products/Nogo-A-Antibody-Immunohistochemistry-Paraffin-NBP2-41071-img0009.jpg "Immunohistochemistry-Paraffin: Nogo-A Antibody - BSA Free [NBP2-41071]")
Immunohistochemistry-Paraffin: Nogo-A Antibody - BSA Free [NBP2-41071]
Immunohistochemistry-Paraffin: Nogo-A Antibody [NBP2-41071] - Mouse brain tissue with NogoA Antibodyat 5 ug/mL.![Immunohistochemistry-Paraffin: Nogo-A Antibody - BSA Free [NBP2-41071]](https://resources.rndsystems.com/images/products/Nogo-A-Antibody-Immunohistochemistry-Paraffin-NBP2-41071-img0008.jpg "Immunohistochemistry-Paraffin: Nogo-A Antibody - BSA Free [NBP2-41071]")
Immunohistochemistry-Paraffin: Nogo-A Antibody - BSA Free [NBP2-41071]
Immunohistochemistry-Paraffin: Nogo-A Antibody [NBP2-41071] - Mouse brain tissue with NogoA antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41071] -
Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41071] - Immunofluorescence of Nogo-A in mouse brain tissue with Nogo-A Antibody at 20 u/mL.Green: Nogo-A antibody
Red: Phylloidin staining
Blue: DAPI staining
Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41071] -
Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41071] - Immunofluorescence of Nogo-A in Mouse Brain tissue with Nogo-A antibody at 20 ug/mL.
Immunohistochemistry: Nogo-A Antibody - BSA Free [NBP2-41071] -
Immunohistochemistry: Nogo-A Antibody - BSA Free [NBP2-41071] - Immunohistochemistry of Nogo-A in mouse brain tissue with Nogo-A antibody at 2.5 ug/mL.Applications for Nogo-A Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Western Blot
0.5 - 1 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nogo-A
Long Name
Reticulon 4A
Alternate Names
NI220, NogoA, RTN4, RTN4A
Gene Symbol
RTN4
Additional Nogo-A Products
Product Documents for Nogo-A Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Nogo-A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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