Non-Human Primate VEGF-A Cartridge

R&D Systems | Catalog # SPCKB-NP-013073

Simple Plex NHP VEGF-A assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.54 pg/mL

Assay Range

3.84-5867 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Non-Human Primate VEGF-A Cartridge Summary

Simple Plex assay is for the detection of non-human primate Vascular Endothelial Growth Factor A (VEGF-A or VEGF) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all VEGF Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Non-human Primate

Specificity

Natural and recombinant VEGF-A.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 38 34
Mean (pg/mL) 44.8 2008 44.1 2096
Standard Deviation 1.60 45.3 4.13 159
CV% 3.6 2.3 9.4 7.6

Recovery for Non-Human Primate VEGF-A Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 106 101-110

Linearity

Samples containing and/or spiked with high concentrations of non-human primate VEGF-A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay. 

Simple Plex Non-Human Primate VEGF-A Assay Linearity

Scientific Data Images for Non-Human Primate VEGF-A Cartridge

Simple Plex Non-Human Primate VEGF-A Assay Standard Curve

Simple Plex Non-Human Primate VEGF-A Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Non-Human Primate VEGF-A Assay. Assay range is 3.84-5867 pg/mL.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

Additional VEGF Products

Product Documents for Non-Human Primate VEGF-A Cartridge

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Certificate of Analysis

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Product Specific Notices for Non-Human Primate VEGF-A Cartridge

For research use only

Citations for Non-Human Primate VEGF-A Cartridge

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