non-muscle Myosin IIA Antibody (2B3) - Azide and BSA Free

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• non-muscle Myosin IIA Antibody (2B3)

  Name: Valentina Lodde

  Application: immunofluorescence on in vitro grown cells

  Sample Tested: granulosa (ovarian) cells plated and grown on coverglassess, ovarian granulosa cells and bovine ovarian granulosa cells

  Species: Bovine

  Verified Customer | Posted 06/22/2018

  Anti Myosin IIA immunofluorescent staining (green). DNA in counterstained with DAPI (blu). Control was obtained by omitting the primary antibody

  Unvalidated Species tested: cow Sample: granulosa (ovarian) cells plated and grown on coverglassess Protocol: fixation: 4% PF for 7 minutes at rt permeabilization: 0.1% Triton-X in PBS for 7 minutes. Blocking: 20% donkey serum, 1% BSA in PBS, 1 hour at rt Primary antibody: Mouse Monoclonal non-muscle Myosin IIA Antibody (2B3) 1:50 or 1:100 in 1% BSA/PBS over night at 4°C Secondary antibody: donkey anti mouse -Alexa 488 (Invitrogen), 1:500 DNA Counterstaining: mounting medium with DAPI Negative CTRL was performed by omitting the primary antibody, images were captured under an epifluorescence microscope using the exposure settings Result: the antibody gives a good signal at both concentration tested

  

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