non-muscle Myosin IIA Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19563

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Bovine (99%), Canine (99%), Chicken (96%), Mouse (98%), Rat (97%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Non-muscle Myosin IIA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human non-muscle Myosin IIA. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (92%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

227 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for non-muscle Myosin IIA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA Antibody [NBP2-19563]

Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA Antibody [NBP2-19563]

Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP2-19563] - Non-muscle Myosin IIA antibody detects Non-muscle Myosin IIA protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Non-muscle Myosin IIA stained by Non-muscle Myosin IIA antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP2-19563]

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP2-19563]

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP2-19563] - Human adenocarcinoma, using MYH9 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563]

Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563]

Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
non-muscle Myosin IIA Antibody

Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] -

Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
non-muscle Myosin IIA Antibody - BSA Free

Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] -

MYH9 antibody [N1], N-term detects MYH9 protein by Western blot analysis.
A. 30 ug NT2D1 whole cell lysate/extract
B. 30 ug PC-3 whole cell lysate/extract
5 % SDS-PAGE
MYH9 antibody [N1], N-term (NBP2-19563) dilution: 1:1000
non-muscle Myosin IIA Antibody - BSA Free

Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] -

MYH9 antibody [N1], N-term detects MYH9 protein by Western blot analysis.
A. 30 ug U87-MG whole cell lysate/extract
B. 30 ug SK-N-SH whole cell lysate/extract
C. 30 ug IMR32 whole cell lysate/extract
D. 30 ug SK-N-AS whole cell lysate/extract
5 % SDS-PAGE
MYH9 antibody [N1], N-term (NBP2-19563) dilution: 1:1000
non-muscle Myosin IIA Antibody - BSA Free

Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term (NBP2-19563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for non-muscle Myosin IIA Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Non-muscle Myosin IIA

This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq]

Long Name

Myosin-9

Alternate Names

MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA

Gene Symbol

MYH9

UniProt

P35579 (Human)

Additional Non-muscle Myosin IIA Products

Product Documents for non-muscle Myosin IIA Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for non-muscle Myosin IIA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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