Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1H2
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Product Specifications
Immunogen
Full length human recombinant protein of human RASSF5 (NP_872606) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
30.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NORE1 Antibody (OTI1H2)
Western Blot: NORE1 Antibody (1H2) [NBP2-03691] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NORE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NORE1.
Flow Cytometry: NORE1 Antibody (OTI1H2) [NBP2-03691]
Flow Cytometry: NORE1 Antibody (1H2) [NBP2-03691] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NORE1 antibody, and then analyzed by flow cytometry.Flow Cytometry: NORE1 Antibody (OTI1H2) [NBP2-03691]
Flow Cytometry: NORE1 Antibody (1H2) [NBP2-03691] - Analysis of Hela cells, using anti-NORE1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).Flow Cytometry: NORE1 Antibody (OTI1H2) [NBP2-03691]
Flow Cytometry: NORE1 Antibody (1H2) [NBP2-03691] - Analysis of Jurkat cells, using anti-NORE1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for NORE1 Antibody (OTI1H2)
Application
Recommended Usage
Flow Cytometry
1:100
Western Blot
1:500
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.41 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NORE1
Alternate Names
Maxp1, MGC10823, MGC17344, New ras effector 1, NORE1A, NORE1B, NORE1RASSF3, Rap1-binding protein, RAPLnovel Ras effector 1, Ras association (RalGDS/AF-6) domain family 5, Ras association (RalGDS/AF-6) domain family member 5, ras association domain-containing protein 5, Ras effector-like protein, regulator for cell adhesion and polarization enriched in lymphoid tissue, Regulator for cell adhesion and polarization enriched in lymphoid tissues, tumor suppressor RASSF3
Entrez Gene IDs
83593 (Human)
Gene Symbol
RASSF5
Additional NORE1 Products
Product Documents for NORE1 Antibody (OTI1H2)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NORE1 Antibody (OTI1H2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Cellular Response to Hypoxia Protocols
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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