NORE1 Antibody (OTI1H2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-73041

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H2

Format

Azide and BSA Free
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Product Specifications

Immunogen

Full length human recombinant protein of human RASSF5 (NP_872606) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NORE1 Antibody (OTI1H2) - Azide and BSA Free

Western Blot: NORE1 Antibody (OTI1H2)Azide and BSA Free [NBP2-73041]

Western Blot: NORE1 Antibody (OTI1H2)Azide and BSA Free [NBP2-73041]

Western Blot: NORE1 Antibody (OTI1H2) - Azide and BSA Free [NBP2-73041] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NORE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NORE1.
Flow Cytometry: NORE1 Antibody (OTI1H2) - Azide and BSA Free [NBP2-73041]

Flow Cytometry: NORE1 Antibody (OTI1H2) - Azide and BSA Free [NBP2-73041]

Flow Cytometry: NORE1 Antibody (OTI1H2) - Azide and BSA Free [NBP2-73041] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-NORE1 antibody, and then analyzed by flow cytometry.
Flow Cytometry: NORE1 Antibody (OTI1H2) - Azide and BSA Free [NBP2-73041]

Flow Cytometry: NORE1 Antibody (OTI1H2) - Azide and BSA Free [NBP2-73041]

Flow Cytometry: NORE1 Antibody (OTI1H2) - Azide and BSA Free [NBP2-73041] - Analysis of Hela cells, using anti-NORE1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: NORE1 Antibody (OTI1H2) - Azide and BSA Free [NBP2-73041]

Flow Cytometry: NORE1 Antibody (OTI1H2) - Azide and BSA Free [NBP2-73041]

Flow Cytometry: NORE1 Antibody (OTI1H2) - Azide and BSA Free [NBP2-73041] - Analysis of Jurkat cells, using anti-NORE1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for NORE1 Antibody (OTI1H2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NORE1

NORE1 (also referred to as Ras Association (RalGDS/AF-6) Domain Family Member 5) is a tumor suppressor and induces apoptosis. NORE1 is known to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D and HIST1H4E. NORE1 has been studied in relation to several types of cancer including lung cancer, breast cancer, clear cell renal cell carcinoma, thyroid carcinoma, and follicular thyroid carcinoma.

Alternate Names

Maxp1, MGC10823, MGC17344, New ras effector 1, NORE1A, NORE1B, NORE1RASSF3, Rap1-binding protein, RAPLnovel Ras effector 1, Ras association (RalGDS/AF-6) domain family 5, Ras association (RalGDS/AF-6) domain family member 5, ras association domain-containing protein 5, Ras effector-like protein, regulator for cell adhesion and polarization enriched in lymphoid tissue, Regulator for cell adhesion and polarization enriched in lymphoid tissues, tumor suppressor RASSF3

Gene Symbol

RASSF5

Additional NORE1 Products

Product Documents for NORE1 Antibody (OTI1H2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NORE1 Antibody (OTI1H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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