Rabbit IgG PE-conjugated Antibody

Catalog # Availability Size / Price Qty
F0110
Detection of RabbitIgG Primary Antibody by Flow Cytometry
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Rabbit IgG PE-conjugated Antibody Summary

Species Reactivity
Rabbit
Specificity
Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of RabbitIgG Primary Antibody by Flow Cytometry View Larger

Detection of RabbitIgG Primary Antibody by Flow Cytometry Mouse splenocytes were stained with Rat Anti-MouseCD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog # FAB7614A)and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (Catalog #MAB6944)or (B) Normal Rabbit IgG Control
(Catalog # AB-105-C)followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody(Catalog # F0110) at 10 μL/106 cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitution default not known
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Rabbit IgG PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A stable and reproducible human blood-brain barrier model derived from hematopoietic stem cells.
    Authors: Cecchelli R, Aday S, Sevin E, Almeida C, Culot M, Dehouck L, Coisne C, Engelhardt B, Dehouck M, Ferreira L
    PLoS ONE, 2014;9(6):e99733.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies.
    Authors: Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A
    , 2014;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC Frozen

FAQs

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Reviews for Rabbit IgG PE-conjugated Antibody

Average Rating: 5 (Based on 2 Reviews)

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Rabbit IgG PE-conjugated Antibody
By Anonymous on 05/01/2018
Application: ELISA Sample Tested: Serum Species: Rabbit

Rabbit IgG PE-conjugated Antibody
By Anonymous on 03/24/2017
Application: Flow Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Flow cytometry analysis of MDA-MB-231 cells incubated with Rabbit anti-Human NFKB p65 antibody (red) or Isotype Control IgG (black), followed by 10 uL/106 cells of PE-conjugated Goat Anti-Rabbit secondary antibody (#F0110).