Key Product Details

Species Reactivity

Rabbit

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Rabbit IgG

Specificity

Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rabbit IgG PE‑conjugated Antibody

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

Mouse splenocytes were stained with Rat Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (FAB7614A) and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (MAB6944) or (B) Normal Rabbit IgG Control (AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) at 10 µL/106 cells.

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

U937 cells were stained with Rabbit Anti-Human IFN alpha beta R1 Monoclonal Antibody (2452, filled histogram) or (B) Normal Rabbit IgG Control (AB-105-C, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) at 10 µL/106 cells.

Applications for Rabbit IgG PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Reviewed Applications

Read 2 reviews rated 5 using F0110 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitution default not known

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Rabbit IgG PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rabbit IgG PE‑conjugated Antibody

For research use only

Citations for Rabbit IgG PE‑conjugated Antibody

Customer Reviews for Rabbit IgG PE‑conjugated Antibody (2)

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Customer Images

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  • Rabbit IgG PE-conjugated Antibody
    Name: Danlee Enzler
    Application: ELISA
    Sample Tested: Serum
    Species: Rabbit
    Verified Customer | Posted 05/01/2018
  • Rabbit IgG PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 03/24/2017
    Flow cytometry analysis of MDA-MB-231 cells incubated with Rabbit anti-Human NFKB p65 antibody (red) or Isotype Control IgG (black), followed by 10 uL/106 cells of PE-conjugated Goat Anti-Rabbit secondary antibody (#F0110).
    Rabbit IgG PE‑conjugated Antibody F0110

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FAQs

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