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Rabbit IgG PE-conjugated Antibody

  (2 citations)
(2 Reviews)     
Datasheet / CoA / SDS
Detection of RabbitIgG Primary Antibody by Flow Cytometry

Flow Cytometry

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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
  • Species Reactivity
    Rabbit
  • Specificity
    Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Rabbit IgG
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry Goat anti-Rabbit IgG Secondary Antibody [Phycoerythrin]
click image to view larger
Detection of RabbitIgG Primary Antibody by Flow Cytometry Mouse splenocytes were stained with Rat Anti-MouseCD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog # FAB7614A)and either (A) Rabbit Anti-Mouse KLRG1 Monoclonal Antibody (Catalog #MAB6944)or (B) Normal Rabbit IgG Control
(Catalog # AB-105-C)followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody(Catalog # F0110) at 10 μL/106 cells.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
 Applications
 Sample Type
  1. Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies.
    Authors: Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A
    , 2014;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Frozen
  2. A stable and reproducible human blood-brain barrier model derived from hematopoietic stem cells.
    Authors: Cecchelli R, Aday S, Sevin E, Almeida C, Culot M, Dehouck L, Coisne C, Engelhardt B, Dehouck M, Ferreira L
    PLoS ONE, 2014;9(6):e99733.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

FAQs

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Average Rating: 5 (Based on 2 reviews)

Have you used Rabbit IgG PE-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Rabbit IgG PE-conjugated Antibody.

We have 2 reviews tested in 2 species: Human, Rabbit.
We have 2 reviews tested in 2 applications: ELISA, Flow Cytometry.

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Excellent
(2)
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ELISA
(1)
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(1)
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Images Ratings Applications Species Reviewed By Date Details
  Excellent
  ELISA Rabbit Anonymous 05/01/2018
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Summary

Application ELISA
Sample Tested Serum
Species Rabbit
Flow Cytometry Rabbit IgG PE-conjugated Antibody F0110 Array F0110
Enlarge 		Excellent
  Flow Human Anonymous 03/24/2017
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Image Details
Flow Cytometry Rabbit IgG PE-conjugated Antibody F0110
Flow Cytometry: Rabbit IgG PE-conjugated Antibody [F0110].

Summary

Application Flow Cytometry
Sample Tested MDA-MB-231 human breast cancer cell line
Species Human

Other Experimental Details

Other Experimental Details Flow cytometry analysis of MDA-MB-231 cells incubated with Rabbit anti-Human NFKB p65 antibody (red) or Isotype Control IgG (black), followed by 10 uL/106 cells of PE-conjugated Goat Anti-Rabbit secondary antibody (#F0110).

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