NOX5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93004

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 661-765 of human NOX5 (NP_078781.3). AEEAQYGRFLELHMYMTSALGKNDMKAIGLQMALDLLANKEKKDSITGLQTRTQPGRPDWSKVFQKVAAEKKGKVQVFFCGSPALAKVLKGHCEKFGFRFFQENF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOX5 Antibody - BSA Free

Western Blot: NOX5 AntibodyAzide and BSA Free [NBP2-93004]

Western Blot: NOX5 AntibodyAzide and BSA Free [NBP2-93004]

Western Blot: NOX5 Antibody [NBP2-93004] - Western blot analysis of extracts of PC-3 cells, using NOX5 antibody (NBP2-93004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
NOX5 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: NOX5 Antibody - Azide and BSA Free [NBP2-93004] -

Immunocytochemistry/ Immunofluorescence: NOX5 Antibody - Azide and BSA Free [NBP2-93004] - Immunofluorescence analysis of A-549 cells using NOX5 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for NOX5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NOX5

NOX5 is a novel NADPH oxidase that generates superoxide and functions as a H+ channel in a Ca(2+)-dependentmanner.(supplied by OMIM)

Long Name

NADPH oxidase 5

Alternate Names

EC 1.6.3.-, MGC149776, MGC149777, NADPH oxidase 5, NADPH oxidase, EF-hand calcium binding domain 5, NOX5A, NOX5B

Gene Symbol

NOX5

Additional NOX5 Products

Product Documents for NOX5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NOX5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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