Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all REST/NRSF Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 800 and 850 of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NRSF Antibody - BSA Free

Immunohistochemistry-Paraffin: NRSF Antibody [NB100-756]

Immunohistochemistry-Paraffin: NRSF Antibody [NB100-756]

Immunohistochemistry-Paraffin: NRSF Antibody [NB100-756] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REST used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: NRSF Antibody [NB100-756]

Immunoprecipitation: NRSF Antibody [NB100-756]

Immunoprecipitation: NRSF Antibody [NB100-756] - Detection of human REST by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REST antibody NB100-756 used for IP at 6 ug per reaction. For blotting immunoprecipitated REST, NB100-756 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for NRSF Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-10 ug/mg lysate
Application Notes
Western Blot Not recommended. Use rabbit anti-REST antibody NB100-757. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: REST/NRSF

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.

Long Name

RE1-Silencing Transcription Factor

Alternate Names

NRSF, XBR

Entrez Gene IDs

5978 (Human)

Gene Symbol

REST

UniProt

Q13127 (Human)

Additional REST/NRSF Products

Product Documents for NRSF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NRSF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NRSF Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for NRSF Antibody - BSA Free

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  • Q: The customer is complaining that the antibody NB100-756 could not detect the target band and there is non-specific band in western blotting. Much detailed result is enclosed with the mail, please check it and give us some help.

    A: Your customer is observing a band at the same size that we observe for NRSF. The molecular weight of larger proteins can be harder to estimate due to poor resolution afforded by molecular weight ladders, and to the greater degree of variability in their migration through SDS-PAGE gels. NRSF is also expressed as a number of different isoforms, which is more than likely what your customer is detecting.

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