NT-4 Antibody (1G12) - Azide and BSA Free

Novus Biologicals | Catalog # H00004909-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G12

Format

Azide and BSA Free
View all NT-4 Primary Antibodies »

Product Specifications

Immunogen

NTF5 (AAH12421, 1 a.a. ~ 210 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLPLPSCSLPILLLFLLPSVPIESQPPPSTLPPFLAPEWDLLSPRVVLSRGAPAGPPLLFLLEAGAFRESAGAPANRSRRGVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA

Specificity

NTF5 - neurotrophin 5 (neurotrophin 4/5) (1G12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NT-4 Antibody (1G12) - Azide and BSA Free

Proximity Ligation Assay: NT-4 Antibody (1G12) [H00004909-M02]

Proximity Ligation Assay: NT-4 Antibody (1G12) [H00004909-M02]

Proximity Ligation Assay: NT-4 Antibody (1G12) [H00004909-M02] - Analysis of protein-protein interactions between BDNF and NTF4. HeLa cells were stained with anti-BDNF rabbit purified polyclonal 1:1200 and anti-NTF4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: NT-4 Antibody (1G12) [H00004909-M02] - Detection limit for recombinant GST tagged NTF4 is 1 ng/ml as a capture antibody.

Applications for NT-4 Antibody (1G12) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NT-4

This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq]

Long Name

Neurotrophin 4

Alternate Names

NT-5, NT4, NTF4, NTF5

Entrez Gene IDs

4909 (Human)

Gene Symbol

NTF4

UniProt

AAH12421 (Human)

Additional NT-4 Products

Product Documents for NT-4 Antibody (1G12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NT-4 Antibody (1G12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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