Nucleoporin NUP85 Antibody

Novus Biologicals | Catalog # NB100-1355

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Nucleoporin NUP85 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ARAIIREGSLEGS corresponding to C-Terminus according to NP_079120.1.

Localization

Nuclear

Specificity

Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Nucleoporin NUP85 Antibody

Western Blot: Nucleoporin NUP85 Antibody [NB100-1355]

Western Blot: Nucleoporin NUP85 Antibody [NB100-1355]

Western Blot: Nucleoporin NUP85 Antibody [NB100-1355] - (1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for Nucleoporin NUP85 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in HeLa lysates (calculated MW of 75 kDa band according to NP_079120).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nucleoporin NUP85

Pericentrin is probably a conserved integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays.

Long Name

Nuclear Pore Complex Protein Nup85

Alternate Names

FROUNT, Nup75, NUP85, PCNT1, Pericentrin-1

Entrez Gene IDs

79902 (Human); 18541 (Mouse)

Gene Symbol

NUP85

UniProt

NP_079120. (Human)

Additional Nucleoporin NUP85 Products

Product Documents for Nucleoporin NUP85 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nucleoporin NUP85 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Monocyte Markers

Citations for Nucleoporin NUP85 Antibody

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