NXF5 Overexpression Lysate

Name: NXF5 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-05947
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-05947

Submit a review

Product Documents

100% Guarantee

Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for NXF5 Overexpression Lysate

NXF5 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC212701

Accession#: NM_032946

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug) and 1 vial of empty vector negative control (100ug). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml).

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate.

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for NXF5 Overexpression Lysate

Western Blot: NXF5 Overexpression Lysate [NBP2-05947]

Western Blot: NXF5 Overexpression Lysate (Adult Normal) [NBP2-05947] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NXF5.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: NXF5

NXF5, also known as Nuclear RNA export factor 5, consists of 6 isoforms ranging from a 168 amino acid isoform that is 20 kDa to a 397 amino acid isoform that is 46 kDa, it is implicated in mRNA nuclear export and could also have a role in polarized cytoplasmic transport and localization of mRNA in neurons. On the other hand, this protein has lost several C-terminal protein domains found in other family members that are required for export activity, and may be an evolving pseudogene. Current research is being performed on several diseases including pelizaeus-merzbacher disease, premature ovarian failure, short stature, intellectual disability, influenza, and neuronitis. The NXF5 has shown an interaction with ALYREF, EIF4A3, NUP98, NUPL2, NXT1, NXT2, RAE1, and THOC5 in ribosome biogenesis in eukaryotes, RNA transport, mRNA surveillance pathway, and influenza A pathways.

Alternate Names

nuclear RNA export factor 5, TAPL1, TAPL-1, TAP-like protein 1

Gene Symbol

NXF5

Additional NXF5 Products

Product Documents for NXF5 Overexpression Lysate

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NXF5 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customer Reviews for NXF5 Overexpression Lysate

There are currently no reviews for this product. Be the first to review NXF5 Overexpression Lysate and earn rewards!

Have you used NXF5 Overexpression Lysate?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image