O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32791

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Porcine (100%), Xenopus (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all O-GlcNAc Transferase/OGT Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human O-GlcNAc Transferase p110 subunit. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (98%).

Localization

Cytoplasm (Potential), Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit O-GlcNAc Transferase p110 subunit Antibody - BSA Free (NBP1-32791) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-O-GlcNAc Transferase p110 subunit Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Scale bar= 10um.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Rat brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody ) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse brain.O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Mouse brain. O-GlcNAc transferase stained by O-GlcNAc transferase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunoprecipitation: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791]

Immunoprecipitation: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 ug of O-GlcNAc transferase antibody. Western blot analysis was performed using O-GlcNAc transferase antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
O-GlcNAc Transferase p110 subunit Antibody

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] -

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - O-GlcNAc Transferase p110 subunit antibody detects O-GlcNAc Transferase p110 subunit protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
O-GlcNAc Transferase p110 subunit stained by O-GlcNAc Transferase p110 subunit antibody (NBP1-32791) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
O-GlcNAc Transferase p110 subunit Antibody

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] -

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - O-GlcNAc Transferase p110 subunit antibody detects O-GlcNAc Transferase p110 subunit protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
O-GlcNAc Transferase p110 subunit stained by O-GlcNAc Transferase p110 subunit antibody (NBP1-32791) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
O-GlcNAc Transferase p110 subunit Antibody

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] -

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - O-GlcNAc Transferase p110 subunit antibody detects O-GlcNAc Transferase p110 subunit protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
O-GlcNAc Transferase p110 subunit stained by O-GlcNAc Transferase p110 subunit antibody (NBP1-32791) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
O-GlcNAc Transferase p110 subunit Antibody

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] -

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] -

O-GlcNAc Transferase p110 subunit antibody detects O-GlcNAc Transferase p110 subunit protein by western blot analysis.
A. 30 ug PC-12 whole cell extract
5% SDS-PAGE
O-GlcNAc Transferase p110 subunit antibody (NBP1-32791) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc Transferase p110 subunit antibody (NBP1-32791) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc Transferase p110 subunit antibody (NBP1-32791) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with O-GlcNAc transferase antibody (NBP1-32791) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Western Blot: O-GlcNAc Transferase p110 subunit Antibody [NBP1-32791] -

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.
A. 30 ug PC-12 whole cell extract
5% SDS-PAGE
O-GlcNAc transferase antibody (NBP1-32791) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: O-GlcNAc Transferase/OGT

O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

O-Linked N-Acetylglucosamine (GlcNAc) Transferase

Alternate Names

HRNT1, OGlcNAc Transferase, OGT

Gene Symbol

OGT

Additional O-GlcNAc Transferase/OGT Products

Product Documents for O-GlcNAc Transferase p110 subunit Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for O-GlcNAc Transferase p110 subunit Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

Citations for O-GlcNAc Transferase p110 subunit Antibody - BSA Free

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Protocols

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