Occludin Antibody (MSVA-415M)

Name: Occludin Antibody (MSVA-415M)
Brand: Novus Biologicals
SKU: NBP3-57255
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-57255

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # MSVA-415M

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Product Specifications

Immunogen

ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary) Swissprot Q16625

Localization

Cell Surface and Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes..

Negative Control
Negitve control:Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Occludin Antibody (MSVA-415M)

Immunohistochemistry-Paraffin:Occludin Antibody (MSVA-415M)NBP3-57255

Clear cell ovarian carcinoma with moderate occludin staining of tumor cells

Immunohistochemistry-Paraffin:Occludin Antibody (MSVA-415M)NBP3-57255

In squamous epithelium occludin positivity is regularly seen but staining is reduced or absent in basal and superficial cell layers scaled

Immunohistochemistry-Paraffin:Occludin Antibody (MSVA-415M)NBP3-57255

Membranous occludin immunostaining of respiratory epithelium scaled

Applications for Occludin Antibody (MSVA-415M)

Application

Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200

Application Notes

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Occludin

The Occludin gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.

Alternate Names

BLCPMG, OCLN

Gene Symbol

OCLN

Additional Occludin Products

Product Documents for Occludin Antibody (MSVA-415M)

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Certificate of Analysis

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Product Specific Notices for Occludin Antibody (MSVA-415M)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.