Occludin Antibody (OCLN/2183)
Novus Biologicals | Catalog # NBP3-07138
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # OCLN/2183
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Product Specifications
Immunogen
Recombinant human Occludin fragment around aa 282-415 (Exact sequence is proprietary) (Uniprot: Q16625)
Localization
Cell Surface and Cytoplasmic
Marker
Tight Junctions Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08880)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Occludin Antibody (OCLN/2183)
![Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2183) [NBP3-07138]](https://resources.rndsystems.com/images/products/Occludin-Antibody-OCLN-2183-Immunohistochemistry-Paraffin-NBP3-07138-img0003.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2183) [NBP3-07138]")
Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2183) [NBP3-07138]
Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2183) [NBP3-07138] - Formalin-fixed, paraffin-embedded human Kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2183).![Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2183) [NBP3-07138]](https://resources.rndsystems.com/images/products/Occludin-Antibody-OCLN-2183-Immunohistochemistry-Paraffin-NBP3-07138-img0002.jpg "Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2183) [NBP3-07138]")
Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2183) [NBP3-07138]
Immunohistochemistry-Paraffin: Occludin Antibody (OCLN/2183) [NBP3-07138] - Formalin-fixed, paraffin-embedded human Kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2183).![Protein Array: Occludin Antibody (OCLN/2183) [NBP3-07138]](https://resources.rndsystems.com/images/products/Occludin-Antibody-OCLN-2183-Protein-Array-NBP3-07138-img0001.jpg "Protein Array: Occludin Antibody (OCLN/2183) [NBP3-07138]")
Protein Array: Occludin Antibody (OCLN/2183) [NBP3-07138]
Protein Array: Occludin Antibody (OCLN/2183) [NBP3-07138] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Occludin Monospecific Mouse Monoclonal Antibody (OCLN/2183).Applications for Occludin Antibody (OCLN/2183)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Occludin
Additional Occludin Products
Product Documents for Occludin Antibody (OCLN/2183)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Occludin Antibody (OCLN/2183)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Blood-Brain Barrier and Immune Cell Transmigration: ICAM-1/CD54 Signaling Pathways
Blood-Brain Barrier and Immune Cell Transmigration: Pathways Overview
Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling Pathways
Blood-Brain Barrier and Immune Cell Transmigration: VEGF Signaling Pathways
