OCT4 Antibody (CL7621) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44315

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL7621

Format

Azide and BSA Free
View all OCT4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q01860, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for OCT4 Antibody (CL7621) - Azide and BSA Free

OCT4 Antibody (CL7621) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: 45934 Antibody [NBP3-44315]

Immunocytochemistry/ Immunofluorescence: 45934 Antibody [NBP3-44315]

Staining of A-431 cells using the Anti-45934 monoclonal antibody).
OCT4 Antibody (CL7621) - Azide and BSA Free Western Blot: 45934 Antibody [NBP3-44315]

Western Blot: 45934 Antibody [NBP3-44315]

Analysis in human cell line NTERA-2.

Applications for OCT4 Antibody (CL7621) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1 ug/ml
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OCT4

The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development.

Alternate Names

class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein

Gene Symbol

POU5F1

Additional OCT4 Products

Product Documents for OCT4 Antibody (CL7621) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for OCT4 Antibody (CL7621) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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