OCT4 Antibody (CL7621) - BSA Free

Novus Biologicals | Catalog # NBP3-21158

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL7621

Format

BSA Free
View all OCT4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q01860, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for OCT4 Antibody (CL7621) - BSA Free

Western Blot: OCT4 Antibody [NBP3-21158] -

Western Blot: OCT4 Antibody [NBP3-21158] -

Analysis in human cell line NTERA-2.
Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NBP3-21158] -

Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NBP3-21158] -

Staining of A-431 cells using the Anti-POU5F1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Applications for OCT4 Antibody (CL7621) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: OCT4

The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development.

Alternate Names

class 5, transcription factor 1, MGC22487, Oct-3, OCT3Oct4, Oct-4, Octamer-binding protein 3, otc-4, OTF3POU domain class 5, transcription factor 1, OTF4, POU class 5 homeobox 1, POU-type homeodomain-containing DNA-binding protein

Gene Symbol

POU5F1

Additional OCT4 Products

Product Documents for OCT4 Antibody (CL7621) - BSA Free

Certificate of Analysis

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Product Specific Notices for OCT4 Antibody (CL7621) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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