Optimedin Overexpression Lysate

Name: Optimedin Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-13927
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBL1-13927

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Optimedin Overexpression Lysate

Optimedin Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC205436

Accession#: NM_058170

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

52.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for Optimedin Overexpression Lysate

Western Blot: Optimedin Overexpression Lysate [NBL1-13927]

Western Blot: Optimedin Overexpression Lysate (Adult Normal) [NBL1-13927] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optimedin.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Optimedin

Optimedin, also known as olfactomedin 3 (OLFM3) and Noelin-3, is a protein expressed in the eyes, brain, and lungs. The protein is known to stimulate the formation of cell adherent and cell-cell tight junctions. Additionally, its expression modulates cell migration and the organization of the cytoskeleton. In one study, cells expressing high levels of optimedin showed higher growth rates and stronger adhesion to the collagen extracellular matrix as compared with control cells. Optimedin has also been identified as a candidate gene for glaucoma.

Alternate Names

NOE3OPTIMEDIN, noelin 3, NOELIN3, noelin-3, NOELIN3_V1, NOELIN3_V2, NOELIN3_V3, NOELIN3_V4, NOELIN3_V5, NOELIN3_V6, olfactomedin 3, olfactomedin related ER localized protein 3, olfactomedin-3, Optimedin

Gene Symbol

OLFM3

Additional Optimedin Products

Product Documents for Optimedin Overexpression Lysate

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Optimedin Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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