OSBPL5 Antibody

Novus Biologicals | Catalog # NB100-57071

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all OSBPL5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence KEEAFLRRRFSLC corresponding to N-Terminus according to NP_065947.1, NP_663613.1, NP_001137535.1.

Epitope

KEEAFLRRRFSLC

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22241858).

Specificity

This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1; NP_001137535.1) encode the same isoform.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for OSBPL5 Antibody

Western Blot: OSBPL5 Antibody [NB100-57071]

Western Blot: OSBPL5 Antibody [NB100-57071]

Western Blot: OSBPL5 Antibody [NB100-57071] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: OSBPL5 Antibody [NB100-57071]

Immunocytochemistry/ Immunofluorescence: OSBPL5 Antibody [NB100-57071]

Immunocytochemistry/Immunofluorescence: OSBPL5 Antibody [NB100-57071] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071]

Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071]

Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071] - Staining of Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: OSBPL5 Antibody [NB100-57071]

Flow Cytometry: OSBPL5 Antibody [NB100-57071]

Flow Cytometry: OSBPL5 Antibody [NB100-57071] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: OSBPL5 Antibody [NB100-57071]

Western Blot: OSBPL5 Antibody [NB100-57071]

Western Blot: OSBPL5 Antibody [NB100-57071] - (0.3ug/ml) staining of Rat Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: OSBPL5 Antibody [NB100-57071]

Immunocytochemistry/ Immunofluorescence: OSBPL5 Antibody [NB100-57071]

Immunocytochemistry/Immunofluorescence: OSBPL5 Antibody [NB100-57071] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071]

Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071]

Immunohistochemistry-Paraffin: OSBPL5 Antibody [NB100-57071] - Staining of Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for OSBPL5 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.03 - 0.3 ug/ml
Application Notes
Western blot: Approx. 100kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4kDa according to Human NP_065947.1 and 101.7kDa according to Rat NP_001015024.2)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: OSBPL5

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Transcript variants encoding different isoforms have been identified.

Alternate Names

EC 1.2.1, EC 2.7.7.6, FLJ42929, KIAA1534oxysterol-binding protein-related protein 5, OBPH1, ORP-5, ORP5oxysterol-binding protein homologue 1, OSBP-related protein 5, oxysterol binding protein-like 5, oxysterol-binding protein homolog 1, Oxysterol-binding protein homolog 1;

Entrez Gene IDs

114879 (Human); 79196 (Mouse)

Gene Symbol

OSBPL5

UniProt

NP_065947 (Human)

Additional OSBPL5 Products

Product Documents for OSBPL5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for OSBPL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for OSBPL5 Antibody

Customer Reviews for OSBPL5 Antibody

There are currently no reviews for this product. Be the first to review OSBPL5 Antibody and earn rewards!

Have you used OSBPL5 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies