OTP Antibody (CL11222)
Novus Biologicals | Catalog # NBP3-52074
Recombinant Monoclonal Antibody
![OTP Antibody (CL11222) Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]](https://resources.rndsystems.com/images/products/nbp3-52074_mouse-otp-mab-cl11222-immunohistochemistry-27320261519619.jpg "Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # CL11222
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q5XKR4, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for OTP Antibody (CL11222)
Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]
Staining of rat hypothalamus shows strong nuclear positivity in a subset of neurons.![OTP Antibody (CL11222) Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]](https://resources.rndsystems.com/images/products/nbp3-52074_mouse-otp-mab-cl11222-immunohistochemistry-273202615275511.jpg "Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]")
Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]
Staining of human fallopian tube shows no positivity in glandular cells as expected.![OTP Antibody (CL11222) Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]](https://resources.rndsystems.com/images/products/nbp3-52074_mouse-otp-mab-cl11222-immunohistochemistry-273202615211917.jpg "Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]")
Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]
Staining of human hypothalamus shows moderate to strong nuclear positivity in a subset of neurons.![OTP Antibody (CL11222) Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]](https://resources.rndsystems.com/images/products/nbp3-52074_mouse-otp-mab-cl11222-immunohistochemistry-273202615293719.jpg "Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]")
Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]
Staining of human lung tumor (typical carcinoid) shows strong nuclear positivity in tumor cells.![OTP Antibody (CL11222) Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]](https://resources.rndsystems.com/images/products/nbp3-52074_mouse-otp-mab-cl11222-immunohistochemistry-27320261519621.jpg "Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]")
Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]
Staining of human lymph node shows no positivity in lymphoid cells as expected.![OTP Antibody (CL11222) Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]](https://resources.rndsystems.com/images/products/nbp3-52074_mouse-otp-mab-cl11222-immunohistochemistry-27320261519248.jpg "Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]")
Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]
Staining of human lung tumor (small cell lung cancer) shows no positivity in tumor cells as expected.![OTP Antibody (CL11222) Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]](https://resources.rndsystems.com/images/products/nbp3-52074_mouse-otp-mab-cl11222-immunohistochemistry-27320261519623.jpg "Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]")
Immunohistochemistry: OTP Antibody (CL11222) [NBP3-52074]
Staining of human cerebellum shows no positivity in Purkinje cells as expected.Applications for OTP Antibody (CL11222)
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OTP
Alternate Names
homeobox protein orthopedia, MGC3161, orthopedia homeobox, orthopedia homolog, orthopedia homolog (Drosophila)
Gene Symbol
OTP
Additional OTP Products
Product Documents for OTP Antibody (CL11222)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for OTP Antibody (CL11222)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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