![Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]](https://resources.rndsystems.com/images/products/OTP-Antibody-Immunohistochemistry-Paraffin-NBP2-68751-img0005.jpg "Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GRQQAMAQSLSQCSLAAGPPPNSMGLSNSLAGSNGAGLQSHLYQPAFPGMVPASLPGPSNVSGSPQLCSSPDSSDVWRGTSIASLRRKALEHTVSMSFT
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for OTP Antibody
Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]
Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751] - Staining of human hypothalamus shows nuclear positivity in neurons.![Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]](https://resources.rndsystems.com/images/products/OTP-Antibody-Immunohistochemistry-Paraffin-NBP2-68751-img0003.jpg "Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]")
Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]
Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751] - Staining of human tonsil shows no positivity in germinal center cells as expected.![Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]](https://resources.rndsystems.com/images/products/OTP-Antibody-Immunohistochemistry-Paraffin-NBP2-68751-img0004.jpg "Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]")
Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]
Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751] - Staining of human liver shows no positivity in hepatocytes as expected.![Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]](https://resources.rndsystems.com/images/products/OTP-Antibody-Immunohistochemistry-Paraffin-NBP2-68751-img0001.jpg "Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]")
Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751]
Immunohistochemistry-Paraffin: OTP Antibody [NBP2-68751] - Immunohistochemical staining of human hypothalamus shows strong nuclear positivity in subsets of neuronal cells.![OTP Antibody Immunohistochemistry: OTP Antibody [OTP Antibody]](https://resources.rndsystems.com/images/products/nbp2-68751_-immunohistochemistry-639204443941370978.jpg "Immunohistochemistry: OTP Antibody [OTP Antibody]")
Immunohistochemistry: OTP Antibody [OTP Antibody]
Staining of human hypothalamus shows nuclear positivity in neurons.![OTP Antibody Immunohistochemistry: OTP Antibody [OTP Antibody]](https://resources.rndsystems.com/images/products/nbp2-68751_-immunohistochemistry-639204443946194413.jpg "Immunohistochemistry: OTP Antibody [OTP Antibody]")
Immunohistochemistry: OTP Antibody [OTP Antibody]
Staining of human tonsil shows no positivity in non-germinal center cells as expected.![OTP Antibody Immunohistochemistry: OTP Antibody [OTP Antibody]](https://resources.rndsystems.com/images/products/nbp2-68751_-immunohistochemistry-639204443954647318.jpg "Immunohistochemistry: OTP Antibody [OTP Antibody]")
Immunohistochemistry: OTP Antibody [OTP Antibody]
Staining of human cerebral cortex shows no positivity in neurons as expected.![OTP Antibody Immunohistochemistry: OTP Antibody [OTP Antibody]](https://resources.rndsystems.com/images/products/nbp2-68751_-immunohistochemistry-639204443956260585.jpg "Immunohistochemistry: OTP Antibody [OTP Antibody]")
Immunohistochemistry: OTP Antibody [OTP Antibody]
Staining of human liver shows no positivity in hepatocytes as expected.Applications for OTP Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
Recommended conditions for IHC,Retrieval method: HIER pH6
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OTP
Alternate Names
homeobox protein orthopedia, MGC3161, orthopedia homeobox, orthopedia homolog, orthopedia homolog (Drosophila)
Gene Symbol
OTP
Additional OTP Products
Product Documents for OTP Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for OTP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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