Otubain-2 Antibody (2E7) - Azide and BSA Free

Novus Biologicals | Catalog # H00078990-M14

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

ELISA, Sandwich ELISA, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E7

Format

Azide and BSA Free
View all Otubain-2 Primary Antibodies »

Product Specifications

Immunogen

OTUB2 (NP_075601, 126 a.a. ~ 234 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH

Specificity

OTUB2 - OTU domain, ubiquitin aldehyde binding 2 (2E7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Novus Biologicals Mouse Otubain-2 Antibody (2E7) - Azide and BSA Free (H00078990-M14) is a monoclonal antibody validated for use in WB, ELISA and IP. Anti-Otubain-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Otubain-2 Antibody (2E7) - Azide and BSA Free

Sandwich ELISA: Otubain-2 Antibody (2E7) [H00078990-M14] - Detection limit for recombinant GST tagged OTUB2 is 1 ng/ml as a capture antibody.
Immunoprecipitation: Otubain-2 Antibody (2E7) [H00078990-M14]

Immunoprecipitation: Otubain-2 Antibody (2E7) [H00078990-M14]

Immunoprecipitation: Otubain-2 Antibody (2E7) [H00078990-M14] - Analysis of OTUB2 transfected lysate using anti-OTUB2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with OTUB2 rabbit polyclonal antibody.

Applications for Otubain-2 Antibody (2E7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is useful for ELISA.WB was previously validated through publication (30241937) for a previous lot. Current lot has been reported by several customers to not work in WB and is not recommended.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Otubain-2

Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond.[supplied by OMIM]

Long Name

OTU Domain, Ubiquitin Aldehyde Binding 2

Alternate Names

C14orf137, OTU2, OTUB2, Otubain2

Entrez Gene IDs

78990 (Human)

Gene Symbol

OTUB2

UniProt

NP_075601 (Human)

Additional Otubain-2 Products

Product Documents for Otubain-2 Antibody (2E7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Otubain-2 Antibody (2E7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for Otubain-2 Antibody (2E7) - Azide and BSA Free

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail