p130 Antibody (2C8A8) - BSA Free

Novus Biologicals | Catalog # NBP2-52551

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C8A8

Format

BSA Free
View all p130 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human p130 (AA: 939-1139) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

128.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p130 Antibody (2C8A8) - BSA Free

Western Blot: p130 Antibody (2C8A8)BSA Free [NBP2-52551]

Western Blot: p130 Antibody (2C8A8)BSA Free [NBP2-52551]

Western Blot: p130 Antibody (2C8A8) [NBP2-52551] - Analysis using RBL2 mAb against human RBL2 (AA: 939-1139) recombinant protein. (Expected MW is 48.7 kDa)
Flow Cytometry: p130 Antibody (2C8A8) - BSA Free [NBP2-52551]

Flow Cytometry: p130 Antibody (2C8A8) - BSA Free [NBP2-52551]

Flow Cytometry: p130 Antibody (2C8A8) [NBP2-52551] - Analysis of Hela cells using RBL2 mouse mAb (green) and negative control (red).
Western Blot: p130 Antibody (2C8A8)BSA Free [NBP2-52551]

Western Blot: p130 Antibody (2C8A8)BSA Free [NBP2-52551]

Western Blot: p130 Antibody (2C8A8) [NBP2-52551] - Analysis using RBL2 mAb against HEK293 (1) and RBL2 (AA: 939-1139)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: p130 Antibody (2C8A8) - BSA Free [NBP2-52551]

ELISA: p130 Antibody (2C8A8) - BSA Free [NBP2-52551]

ELISA: p130 Antibody (2C8A8) [NBP2-52551] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for p130 Antibody (2C8A8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p130

Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.

Alternate Names

FLJ26459, p130, pRb2, RB2, RBR-2, retinoblastoma-like 2 (p130), retinoblastoma-like protein 2,130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2

Gene Symbol

RBL2

Additional p130 Products

Product Documents for p130 Antibody (2C8A8) - BSA Free

Certificate of Analysis

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Product Specific Notices for p130 Antibody (2C8A8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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