p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80897

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Golden Syrian Hamster (Negative)

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 5-C3-1

Format

Azide and BSA Free
View all p19ARF/CDKN2A Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide containing amino acids 54-75 of the murine p19ARF/CDKN2A. [UniProt# Q64364]

Epitope

The epitope recognized is between amino acids 62-75 of murine p19ARF/CDKN2A.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 27177758). The antibody does not react with Golden Syrian hamster. Armenian hamster has not been tested.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free

Western Blot: p19ARF/CDKN2A Antibody (5-C3-1)Azide and BSA Free [NBP2-80897]

Western Blot: p19ARF/CDKN2A Antibody (5-C3-1)Azide and BSA Free [NBP2-80897]

Western Blot: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free [NBP2-80897] - Whole cell protein from mouse Neuro2A and MEF cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-p19ARF in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free [NBP2-80897]

Immunocytochemistry/ Immunofluorescence: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free [NBP2-80897]

Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free [NBP2-80897] - Detection of p19Arf. Wild type mouse embryo fibroblasts (MEF) at passage 5 (panels 1, 2) and NIH3T3 cells, which have deleted the Arf gene (panels 3, 4) were probed with NB200-17. Image from the standard format of this antibody.
Immunohistochemistry: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free [NBP2-80897]

Immunohistochemistry: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free [NBP2-80897]

Immunohistochemistry: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free [NBP2-80897] - Staining of sections from a wild type mouse testis taken at P11 were probed with NB200-174 (green). Sections were stained with DAPI to reveal the position of cell nuclei (blue). Image from the standard format of this antibody.
Western Blot: p19ARF/CDKN2A Antibody (5-C3-1)Azide and BSA Free [NBP2-80897]

Western Blot: p19ARF/CDKN2A Antibody (5-C3-1)Azide and BSA Free [NBP2-80897]

Western Blot: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free [NBP2-80897] - Analysis of endogenous p19Arf. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE. Image from the standard format of this antibody.
Western Blot: p19ARF/CDKN2A Antibody (5-C3-1)Azide and BSA Free [NBP2-80897]

Western Blot: p19ARF/CDKN2A Antibody (5-C3-1)Azide and BSA Free [NBP2-80897]

Western Blot: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free [NBP2-80897] - Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (Lanes 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (Lanes 3), or NB200-174 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analysed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) from Arf null NIH3T3 cells (Lanes 1) and HA- p19Arf expressing cells (Lanes 2) were also analysed by Western blotting, as input controls. Image from the standard format of this antibody.
p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free

Flow Cytometry: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free

Flow Cytometry: p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free - Conducted the antibody trial with two different dilutions of p19ARF/CDKN2A Antibody (5-C3-1) [PE] (Catalog # NB200-174PE) (1:500 and 1:100) and compared them to an IgG control which was also PE labelled. Image from a verified customer review.

Applications for p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/ml

Immunocytochemistry/ Immunofluorescence

1:500 - 1:1000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Frozen

5 ug/ml

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 27177758)

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500 - 1:1000
Application Notes
In Western blot a band can be seen at ~19 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p19ARF/CDKN2A

ARF (alternative reading frame; p14ARF in human, p19ARF in mouse) is a tumor suppressor that serves as a sensor of hyper-proliferative signals, resulting in p53-dependent growth arrest and apoptotic cell death. ARF interacts with several proteins including COMMD1, CDKN2AIP, TBRG1, E4F1, C1QBP etc. and can bind to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI as well as UBE2I/UBC9. ARF has the capability of inducing cell cycle arrest in G1 as well as G2 phases. While ARF is not expressed at significant levels in most normal tissues, oncogene activation triggers ARF overexpression, resulting in inhibition of MDM2 ubiquitin ligase and stabilization of p53. It is induced by progesterone as well as Ras/DMTF1, and is downregulated through NFkB p65 subunit (RELA). Inhibition of the p53 pathway, most commonly via mutations in p53 itself, inactivation of ARF, or amplification of MDM2 has been proposed to be a crucial step in pathogenesis of most human malignancies. Moreover, mice with null ARF have been shown to develop tumors rapidly and to display delayed mammary gland involution.

Alternate Names

alternative reading frame, ARF, ARF-INK4a, CDK4I, CDKN2A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4), cyclin-dependent kinase inhibitor 2A, isoform 3, cyclin-dependent kinase inhibitor 2A, isoforms 1/2, cyclin-dependent kinase inhibitor protein, INK4, INK4a, Ink4a/Arf, INK4a-ARF, mitochondrial smARF', MTS1, p14, p14ARF, P16, p16(INK4a), p16-INK4, P16INK4A, p16-INK4a, p19, p19, p19ARF, Pctr1

Gene Symbol

Cdkn2a

Additional p19ARF/CDKN2A Products

Product Documents for p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for p19ARF/CDKN2A Antibody (5-C3-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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