p23/PTGES3 Antibody (10M9W8)

Novus Biologicals | Catalog # NBP3-16586

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10M9W8 expressed in HEK293
View all p23/PTGES3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human p23/PTGES3 (NP_006592.3). MQPASAKWYDRRDYVFIEFCVEDSKDVNVNFEKSKLTFSCLGGSDNFKHLNEIDLFHCIDPNDSKHKRTDRSILCCLRKGESGQSWPRLTKERAKLNWLS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p23/PTGES3 Antibody (10M9W8)

Western Blot: p23/PTGES3 Antibody (10M9W8) [NBP3-16586]

Western Blot: p23/PTGES3 Antibody (10M9W8) [NBP3-16586]

Western Blot: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Western blot analysis of extracts of various cell lines, using p23/PTGES3 Rabbit mAb (NBP3-16586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586]

Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586]

Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Immunofluorescence analysis of U-2 OS cells using p23/PTGES3 Rabbit mAb (NBP3-16586) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: p23/PTGES3 Antibody (10M9W8) [NBP3-16586]

Western Blot: p23/PTGES3 Antibody (10M9W8) [NBP3-16586]

Western Blot: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Western blot analysis of extracts of various cell lines, using p23/PTGES3 Rabbit mAb (NBP3-16586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586]

Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586]

Immunocytochemistry/Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Immunofluorescence analysis of C6 cells using p23/PTGES3 Rabbit mAb (NBP3-16586) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
p23/PTGES3 Antibody (10M9W8)

Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] -

Immunocytochemistry/ Immunofluorescence: p23/PTGES3 Antibody (10M9W8) [NBP3-16586] - Immunofluorescence analysis of NIH-3T3 cells using p23/PTGES3 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for p23/PTGES3 Antibody (10M9W8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p23/PTGES3

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP 70 and HSP 90), immunosuppressant binding proteins called immunophilins (the FK 506 binding proteins, FKBP 52 & FKBP 54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP 70, HSP 90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP 90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

Long Name

Prostaglandin E Synthase 3 (Cytosolic)

Alternate Names

CPGES, PTGES3, TEBP

Gene Symbol

PTGES3

Additional p23/PTGES3 Products

Product Documents for p23/PTGES3 Antibody (10M9W8)

Certificate of Analysis

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Product Specific Notices for p23/PTGES3 Antibody (10M9W8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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