Human p27/Kip1 ELISA Kit (Colorimetric)

Name: Human p27/Kip1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-66683
Price: 759 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-66683

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Saliva, Tissue Homogenate (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human P27 in samples. No significant cross-reactivity or interference between human P27 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.09%

Inter-Assay Precision (Precision between assays) CV% 5.1%

Recovery for Human p27/Kip1 ELISA Kit (Colorimetric)

Recovery

86-105%

Linearity

Scientific Data Images for Human p27/Kip1 ELISA Kit (Colorimetric)

ELISA: Human p27/Kip1 ELISA Kit (Colorimetric) [NBP2-66683] -

ELISA: Human p27/Kip1 ELISA Kit (Colorimetric) [NBP2-66683] - Standard Curve Reference

Kit Contents for Human p27/Kip1 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for Human p27/Kip1 ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual - Human p27/Kip1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Human p27/Kip1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Autophagy

Cancer Biomarkers

Cell Cycle

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