Mouse p27/Kip1 ELISA Kit (Colorimetric)

Name: Mouse p27/Kip1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-75208
Price: 759 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-75208

Submit a review

Product Documents

100% Guarantee

Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

4.69 pg/mL

Assay Range

7.81 - 500 pg/mL

View all p27/Kip1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This kit recognizes Mouse CDKN1B in samples. No significant cross-reactivity or interference between Mouse CDKN1B and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.24%

Inter-Assay Precision (Precision between assays) CV% 4.43%

Recovery for Mouse p27/Kip1 ELISA Kit (Colorimetric)

Recovery

87-109%

Linearity

Scientific Data Images for Mouse p27/Kip1 ELISA Kit (Colorimetric)

ELISA: Mouse p27/Kip1 ELISA Kit (Colorimetric) [NBP2-75208] -

ELISA: Mouse p27/Kip1 ELISA Kit (Colorimetric) [NBP2-75208] - Standard Curve Reference

Kit Contents for Mouse p27/Kip1 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: p27/Kip1

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for Mouse p27/Kip1 ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual - Mouse p27/Kip1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Mouse p27/Kip1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Autophagy

Cancer Biomarkers

Cell Cycle

Customer Reviews for Mouse p27/Kip1 ELISA Kit (Colorimetric)

There are currently no reviews for this product. Be the first to review Mouse p27/Kip1 ELISA Kit (Colorimetric) and earn rewards!

Have you used Mouse p27/Kip1 ELISA Kit (Colorimetric)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image