P2X5/P2RX5 Antibody (1C5) - Azide and BSA Free

Novus Biologicals | Catalog # H00005026-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C5

Format

Azide and BSA Free
View all P2X5/P2RX5 Primary Antibodies »

Product Specifications

Immunogen

P2RX5 (NP_002552, 126 a.a. ~ 224 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DGACSKDSDCHAGEAVTAGNGVKTGRCLRRENLARGTCEIFAWCPLETSSRPEEPFLKEAEDFTIFIKNHIRFPKFNFSKSNVMDVKDRSFLKSCHFGP

Localization

Integral membrane protein.

Specificity

P2RX5 - purinergic receptor P2X, ligand-gated ion channel, 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for P2X5/P2RX5 Antibody (1C5) - Azide and BSA Free

Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01]

Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01]

Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Analysis of P2RX5 expression in transfected 293T cell line by P2RX5 monoclonal antibody (M01), clone 1C5.Lane 1: P2RX5 transfected lysate(46.42 KDa).Lane 2: Non-transfected lysate.
Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01]

Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01]

Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Analysis of P2RX5 over-expressed 293 cell line, cotransfected with P2RX5 Validated Chimera RNAi ( Cat # H00005026-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with P2RX5 monoclonal antibody (M01), clone 1C5 (Cat # H00005026-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Detection limit for recombinant GST tagged P2RX5 is approximately 1ng/ml as a capture antibody.

Applications for P2X5/P2RX5 Antibody (1C5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: P2X5/P2RX5

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Several characteristic motifs of ATP-gated channels are present in its primary structure, but, unlike other members of the purinoceptors family, this receptor has only a single transmembrane domain. Three transcript variants encoding distinct isoforms have been identified for this gene.

Long Name

P2X Purinergic Receptor 5

Alternate Names

LRH-1, P2RX5

Entrez Gene IDs

5026 (Human)

Gene Symbol

P2RX5

OMIM

602836 (Human)

UniProt

NP_002552

Additional P2X5/P2RX5 Products

Product Documents for P2X5/P2RX5 Antibody (1C5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for P2X5/P2RX5 Antibody (1C5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for P2X5/P2RX5 Antibody (1C5) - Azide and BSA Free

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