P2X5/P2RX5 Antibody (HL2773)
Novus Biologicals | Catalog # NBP3-48730
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2773
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Product Specifications
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human P2X5/P2RX5. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for P2X5/P2RX5 Antibody (HL2773)
Western Blot: P2X5/P2RX5 Antibody (HL2773) [NBP3-48730] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2X5/P2RX5 antibody [HL2773] (NBP3-48730) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: P2X5/P2RX5 Antibody (HL2773) [NBP3-48730] -
P2X5/P2RX5 antibody [HL2773] detects P2X5/P2RX5 protein by immunofluorescent analysis.Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: P2X5/P2RX5 stained by P2X5/P2RX5 antibody [HL2773] (NBP3-48730) diluted at 1:100.
Blue: Fluoroshield with DAPI.
Western Blot: P2X5/P2RX5 Antibody (HL2773) [NBP3-48730] -
Boiled and unboiled Raji whole cell extracts and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2X5/P2RX5 antibody [HL2773] (NBP3-48730) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibodyApplications for P2X5/P2RX5 Antibody (HL2773)
Application
Recommended Usage
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: P2X5/P2RX5
Long Name
P2X Purinergic Receptor 5
Alternate Names
LRH-1, P2RX5
Gene Symbol
P2RX5
Additional P2X5/P2RX5 Products
Product Documents for P2X5/P2RX5 Antibody (HL2773)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for P2X5/P2RX5 Antibody (HL2773)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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