p57 Kip2 Antibody (KIP2/7187)

Novus Biologicals | Catalog # NBP3-20437

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Kappa Clone # KIP2/7187
View all p57 Kip2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human p57 Kip2 protein

Localization

Nucleus.

Specificity

Recognizes a protein of 57kDa, identified as p57 Kip2 It shows no cross-reaction with p27Kip1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG Kappa

Description

Positive Controls: Raji, HT29 or SK-BR3 cells. Human colon or prostate carcinomas.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for p57 Kip2 Antibody (KIP2/7187)

p57 Kip2 Antibody (KIP2/7187)

p57 Kip2 Antibody (KIP2/7187)

IHC analysis of formalin-fixed, paraffin-embedded human complete mole (CM). Strong nuclear staining using p57 Kip2 antibody (KIP2/7187) at 2ug/ml in PBS for 30min RT.
p57 Kip2 Antibody (KIP2/7187)

p57 Kip2 Antibody (KIP2/7187)

IHC analysis of formalin-fixed, paraffin-embedded human placenta. Strong nuclear staining using p57 Kip2 antibody (KIP2/7187) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

Applications for p57 Kip2 Antibody (KIP2/7187)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p57 Kip2

p57kip2 is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate.

Alternate Names

Beckwith-Wiedemann syndrome, BWS, cyclin-dependent kinase inhibitor 1C, cyclin-dependent kinase inhibitor 1C (p57, Kip2), Cyclin-dependent kinase inhibitor p57, KIP2BWCR, p57, p57Kip2, WBS

Gene Symbol

CDKN1C

Additional p57 Kip2 Products

Product Documents for p57 Kip2 Antibody (KIP2/7187)

Certificate of Analysis

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Product Specific Notices for p57 Kip2 Antibody (KIP2/7187)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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