p66 alpha Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30190

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all p66 alpha Primary Antibodies »

Product Specifications

Immunogen

between 225 and 275

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for p66 alpha Antibody - BSA Free

p66 alpha Antibody

Western Blot:[NBP3-30190]-

Western Blot:[NBP3-30190]-Detection of human p66alpha by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit antibody used for WB at 0. 1 µg/ml and 1 µg/ml and used for IP at 10 µg/mg lysate. p66alpha was also immunoprecipitated by rabbit antibodies and, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.

Applications for p66 alpha Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p66 alpha

Methylation of cytosine at CpG dinucleotides is a common feature of many higher eukaryotic genomes (1-3). Increasing evidence indicates that recruitement of histone deacetylase complexes by methyl-CpG-binding proteins (MeCP) is a major mechanism of methylated DNA silencing. P66, a zinc finger-containing protein is one of the components of the MeCP1 complex (4). P66 and p68, the two components of MeCP1 are different forms of the same zinc finger-containing protein and are conserved from C.elegans to humans. Homologs of p66 from different organisms revealed two highly conserved regions, CR1 and CR2. While CR1 is involved in the association of p66 with other MeCP1 components, CR2 plays an important role in targeting p66 and MBD3 to specific loci (4).

Alternate Names

FLJ20085, FLJ21017, GATA zinc finger domain containing 2A, GATA zinc finger domain-containing protein 2A, Hp66alpha, p66 alpha, p66alpha, transcriptional repressor p66-alpha

Gene Symbol

GATAD2A

Additional p66 alpha Products

Product Documents for p66 alpha Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for p66 alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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