p73 Antibody (5B429) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-33259

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B429

Format

Azide and BSA Free
View all p73 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.

Specificity

p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for p73 Antibody (5B429) - Azide and BSA Free

Western Blot: p73 Antibody (5B429)Azide Free [NBP2-33259]

Western Blot: p73 Antibody (5B429)Azide Free [NBP2-33259]

Western Blot: p73 Antibody (5B429) - Azide Free [NBP2-33259] - Analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate at 2 ug/ml. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: p73 Antibody (5B429) - Azide Free [NBP2-33259]

Immunocytochemistry/ Immunofluorescence: p73 Antibody (5B429) - Azide Free [NBP2-33259]

Immunocytochemistry/Immunofluorescence: p73 Antibody (5B429) - Azide Free [NBP2-33259] - p73 was detected in immersion fixed Hela human cell line using p73 [5B429] Monoclonal Antibody (Catalog # NBP2-24737) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and in the nuclei. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: p73 Antibody (5B429) - Azide Free [NBP2-33259]

Immunohistochemistry-Paraffin: p73 Antibody (5B429) - Azide Free [NBP2-33259]

Immunohistochemistry-Paraffin: p73 Antibody (5B429) - Azide Free [NBP2-33259] - FFPE breast cancer probed with p73 antibody at 5 ug/ml. Human tissue was used for this test. Staining of tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Image from t
Flow Cytometry: p73 Antibody (5B429) - Azide Free [NBP2-33259]

Flow Cytometry: p73 Antibody (5B429) - Azide Free [NBP2-33259]

Flow Cytometry: p73 Antibody (5B429) - Azide Free [NBP2-33259] - Detection of p73 in Human HeLa Cell Line. Human HeLa cell line was stained with Mouse Anti- p73 Monoclonal Antibody (Catalog # NBP2-24737, filled histogram), or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a Bio-Techne brand. Image from the standard format of this antibody.
Simple Western: p73 Antibody (5B429)Azide Free [NBP2-33259]

Simple Western: p73 Antibody (5B429)Azide Free [NBP2-33259]

Simple Western: p73 Antibody (5B429) - Azide Free [NBP2-33259] - Lane view shows a specific band for p73 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from the standard format of this antibody.

Applications for p73 Antibody (5B429) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Frozen

5-15 ug/ml

Immunohistochemistry-Paraffin

5-15 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

2 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p73

p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over expression (2) Unlike p53, p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63, p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (alpha, beta, and gamma) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5).

Long Name

Tumor Protein p73

Alternate Names

TP73

Gene Symbol

TP73

Additional p73 Products

Product Documents for p73 Antibody (5B429) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for p73 Antibody (5B429) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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